Competitive Microtubule Binding of PEX14 Coordinates Peroxisomal Protein Import and Motility
نویسندگان
چکیده
منابع مشابه
Structural basis for competitive interactions of Pex14 with the import receptors Pex5 and Pex19.
Protein import into peroxisomes depends on a complex and dynamic network of protein-protein interactions. Pex14 is a central component of the peroxisomal import machinery and binds the soluble receptors Pex5 and Pex19, which have important function in the assembly of peroxisome matrix and membrane, respectively. We show that the N-terminal domain of Pex14, Pex14(N), adopts a three-helical fold....
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Peroxisomes are essential organelles responsible for many metabolic reactions, such as the oxidation of very long chain and branched fatty acids, D-amino acids and polyamines, as well as the production and turnover of hydrogen peroxide. They comprise a class of organelles called microbodies, including glycosomes, glyoxysomes and Woronin bodies. Dysfunction of human peroxisomes causes severe and...
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oxisomal matrix proteins are imported into the organelle from their site of synthesis on free cytoplasmic ribo-somes. This observation led to the classical model of peroxisome proliferation in which existing peroxisomes Amherst, Massachusetts 01003 increase in size via the direct import of proteins from the cytoplasm, and divide by fission or budding (La-zarow and Fujiki, 1985). As such, the bi...
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We have established a procedure for isolating native peroxisomal membrane protein complexes from cultured human cells. Protein-A-tagged peroxin 14 (PEX14), a central component of the peroxisomal protein translocation machinery was genomically expressed in Flp-In-293 cells and purified from digitonin-solubilized membranes. Size-exclusion chromatography revealed the existence of distinct multimer...
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Pex14p, an S. cerevisiae peroxin, is attached to the outer face of the peroxisomal membrane and is a component of the protein import machinery. Pex14p interacts with both the PTS1 and PTS2 receptors. It is the only known peroxisomal membrane protein that binds the PTS2 receptor and might thus mediate the membrane docking event of PTS2-dependent protein import. These results suggest that the two...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2021
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2020.166765